We offer a standalone version, available here, for predicting structures locally. TCR-pMHC Complex: Class I TCR-pMHC Complex: Class II Unbound TCR Enter sequence Select from gene Enter TCR α sequence Enter TCR β sequence Enter peptide sequence Species Human Mouse Enter MHC I sequence or select gene from above As an alternative to entering sequences, you can upload a FASTA file instead. Make sure to use the correct sequence order indicated here. FASTA input: FASTA example: FASTA input Advanced options Perform Amber energy minimization Click to view lists of class I and class II pMHC templates. pMHC PDB templates to exclude: pMHC PDB templates to include: PDB templates date cutoff: Example input: TCR / HLA-A*01 - SARS-CoV-2 ORF3 peptide complex (reference) Example output: TCR / HLA-A*01 - SARS-CoV-2 ORF3 peptide complex Select TCR α sequence from gene Species Human Mouse Generate sequence Select TCR β sequence from gene Species Human Mouse Generate sequence Enter peptide sequence Species Human Mouse Enter MHC I sequence or select gene from above Advanced options Perform Amber energy minimization Click to view lists of class I and class II pMHC templates. pMHC PDB templates to exclude: pMHC PDB templates to include: PDB templates date cutoff: Example input: TCR / HLA-A*01 - SARS-CoV-2 ORF3 peptide complex (reference) Example output: TCR / HLA-A*01 - SARS-CoV-2 ORF3 peptide complex Enter sequence Select from gene Enter TCR α sequence Enter TCR β sequence Enter peptide sequence Species Human Mouse Enter MHC II α sequence or select gene from above Enter MHC II β sequence or select gene from above As an alternative to entering sequences, you can upload a FASTA file instead. Make sure to use the correct sequence order indicated here. Upload a FASTA file: FASTA example: Example fasta input Advanced options Perform Amber energy minimization Click to view lists of class I and class II pMHC templates. pMHC PDB templates to exclude: pMHC PDB templates to include: PDB templates date cutoff: Example input: 4285 TCR - R175H neoantigen peptide complex (reference) Example output: 4285 TCR - R175H neoantigen peptide complex Select TCR α sequence from gene Species Human Mouse Generate sequence Select TCR β sequence from gene Species Human Mouse Generate sequence Enter peptide sequence Species Human Mouse Enter MHC II α sequence or select gene from above Enter MHC II β sequence or select gene from above Advanced options Perform Amber energy minimization Click to view lists of class I and class II pMHC templates. pMHC PDB templates to exclude: pMHC PDB templates to include: PDB templates date cutoff: Example input: 4285 TCR - R175H neoantigen peptide complex (reference) Example output: 4285 TCR - R175H neoantigen peptide complex Enter TCR sequence Select from Germline gene Enter TCR α sequence Enter TCR β sequence As an alternative to entering sequences, you can upload a FASTA file instead. Make sure to use the correct sequence order indicated here. Upload a FASTA file: FASTA example: Example fasta input Advanced options Perform Amber energy minimization PDB templates date cutoff: Example input: BV9–CDR3β TCR (reference) Example output: BV9–CDR3β TCR Select TCR α sequence from gene Species Human Mouse Generate sequence Select TCR β sequence from gene Species Human Mouse Generate sequence Advanced options Perform Amber energy minimization PDB templates date cutoff: Example input: BV9–CDR3β TCR (reference) Example output: BV9–CDR3β TCR